Figure 2.

Distribution of the magnitude of sex bias (log₂ sex expression ratio) is different for male- and female-biased genes. (A) Differential expression was determined using DESeq, q-value <0.05. Only genes with FPKM >1 in at least one sex were considered. Male and female expression was determined using cufflinks and reported as FPKM. Magnitude of sex bias was determined by taking the log₂ of the sex expression ratio. For each category, male or female, genes were binned by magnitude of bias. Percentage of differentially expressed genes contained within each bin is plotted on the y-axis. (B) For C. elegans (fog-2) the magnitude of sex-biased expression is plotted against the level of expression in males (left) and females (right). Dashed line indicates FPKM = 1. Percentages of high-sex-biased genes with low expression (FPKM <1) in the opposite sex are indicated. Nonbiased genes are plotted in black. Male-biased genes are plotted in blue; female-biased genes are plotted in pink. Darker colors indicate high magnitude of bias. R² values between magnitude of bias and expression levels are indicated for each category.