Figure 3.

All subcongenic founder, non-sex-reversed B6-Y^POS male mice had a non-B6 region encompassing Sox9 and its promoter that is not of B6 or 129 origin. (A) Each schematic represents a Y^POS male used for breeding and generation of subcongenic lines and is labeled for each version of chromosome 11 that the animal has (e.g., Line11-1/11-2 is an animal with two different copies of chromosome 11, Chr11-1 and Chr11-2 (see Figure 2B for notations). All of the subcongenic XY^POS breeding males were either heterozygous (green SNPs) or homozygous (blue SNPs) for a large 4.5-Mb region, which encompasses Sox9 and its promoter (red box). (Top) Ideogram of mouse chromosome 11. (B) Diversity array analysis of the 129S1 inbred strain has a SNP profile (black) that has a different pattern from the SNPs in the subcongenic protected males (compare to A). This shows that the congenic regions were not derived from the 129S1 strain, as previously described.