Skip to main content
. 2014 Jul 14;9(7):e99792. doi: 10.1371/journal.pone.0099792

Figure 4. Quantitation of SERPINB12 mRNA during regression and recrudescence of the magnum of laying hens during induced molting.

Figure 4

Quantitative PCR was conducted using cDNA templates from the magnum of hens fed a control diet (Day 0), zinc fed hens in which the oviduct was regressing (Day 6 and 12) [A], and hens in which the oviduct was undergoing recrudescence (Day 20, 25, 30, and 35) [B]. These experiments were performed in triplicate and normalized to control GAPDH expression. The asterisks denote statistically significant differences (mean ± SEM; ** P<0.01 or *** P<0.001).