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. 2014 Jul 14;9(7):e98903. doi: 10.1371/journal.pone.0098903

Figure 1. Flow cytometric of sperm viability using 7-amino-actinomycin-D Dye.

Figure 1

Histograms show: (A) Negative control with 10% 7-AAD positive cells. (B) Positive control with 98.5% 7-AAD positive cells. (C) Semen sample of one male partner of infertile couples positive for C. trachomatis qPCR with 56.5% 7-AAD negative cells and 43.5% 7-AAD positive cells. B: window adjusted to detect the percentage of cells with 7-AAD positive.