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. 2014 Jul 14;9(7):e98903. doi: 10.1371/journal.pone.0098903

Figure 4. TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labeling (TUNEL) assay of spermatozoa.

Figure 4

Histograms show: (A) negative control with 2.35% TUNEL positive cells. (B) Positive control (spermatozoa treated with DNaseI) with 90.5% TUNEL positive cells. (C) Semen sample of one male partner of infertile couples positive for C. trachomatis qPCR with 20.5% TUNEL positive cells. B: window adjusted to detect the percentage of TUNEL positive cells.