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. 2014 Jul 14;9(7):e98903. doi: 10.1371/journal.pone.0098903

Figure 5. Distributions of percentages of different apoptotic markers among patients positive for C. trachomatis qPCR compared to uninfected patients.

Figure 5

(A) Mean percentage of Sperm Vitality, evaluated with 7-amino-actinomycin-D Dye (7-AAD). (B) Mean percentage of Sperm mitochondrial membrane potential (ΔΨm), evaluated with JC-1. (C) Mean percentage of Caspase 3 activation, evaluated with fluorescein-labeled inhibitor of caspases (FLICA). (D) Mean percentage of Sperm DNA fragmentation, evaluated with (TUNEL). Uninfected: Sperm of uninfected patients (negative for all PCRs performed and for spermioculture analysis). CT+: sperm of patients positive for C. trachomatis qPCR. * Indicates significant differences compared with uninfected semen (P<0.05). ** Indicates significant differences compared with uninfected semen (P<0.001).