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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Exp Cell Res. 2013 Dec 12;321(2):123–132. doi: 10.1016/j.yexcr.2013.12.003

Fig. 2.

Fig. 2

RNAi Mediated AHSG Subline Generation from the SQ20B HNSCC Cell Line. (A) Parental SQ20B cells were transduced with empty vector (EV) or small hairpin RNAs (AH50 and AH20) targeting distinct regions of the coding sequence of AHSG. Stable puromycin-selected clones were expanded and the extent of AHSG depletion assessed by immunoblotting using antibodies against AHSG. (B) Densitometric analysis of AHSG expression of AHSG-depleted SQ20B sublines. AHSG expression levels were normalized to GAPDH. Bars represent AHSG expression ± SD from three independent experiments relative to the expression level in the parental SQ20B cells.