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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Exp Cell Res. 2013 Dec 12;321(2):123–132. doi: 10.1016/j.yexcr.2013.12.003

Fig. 6.

Fig. 6

AHSG protects MMP-9 from autolytic degradation in SQ20B HNSCC sublines EV, AH50 and AH20. EV, AH50, and AH20 were cultured in serum-free media for 96 h. (A) Conditioned medium from each cell line was collected and 6 ml of each was used for ultrafiltration to a final volume of 100 µl. Equal concentrations were dissolved in zymography sample buffer and analyzed. Serum-free EV conditioned medium was used as the control. (B) Densitometric quantitation of the zymogram for MMP-9 in (A). (C) MMP- 9 protein in the concentrated conditioned medium from EV, AH50 and AH20 was quantitated by immunoblot using antibodies to MMP-9. MMP-9 bands were normalized to GAPDH and depicted in (D).