Figure 3.

Hematopoietic and nonhematopoietic cells are well distinguished during wound healing in VavR double transgenic mice. (A, B): Histological images and quantification during wound healing show GFP+ hematopoietic cells peaking at day 7, while RFP+ nonhematopoietic cells peak at day 14. Scale bar =50 μm. Quantification includes number of RFP+ and GFP+ cells per high power field (hpf). (C): Flow cytometry demonstrates that majority of the GFP+ cells in the wound were CD45+ (numbers relative to total GFP+ cells). (C–E): RFP+/CD31+ endothelial cells are seen to peak in the wound at day 14 by flow cytometry (C) (percentage relative to total RFP+ cells), and quantification of immunostaining (D, E). Blue: CD31. White arrows highlight areas of RFP/CD31 colocalization. Scale bar =100 μm. Of note, RFP and GFP percentages in (C) are relative numeric values (i.e., RFP counts vs. RFP +GFP counts), and do not reflect absolute cell number changes. Abbreviations: GFP, green fluorescent protein; RFP, red fluorescent protein.