Figure 5.

Single-cell transcriptional analysis reveals importance of monocyte/macrophage lineage cells and their expression of collagen in wound healing. (A): Partitional clustering of green fluorescent protein+ cells in the wound. Columns represent individual cells and rows correspond to genes. Yellow indicates increased expression and blue decreased expression. Monocyte/macrophage lineage cells are characterized by high expression of CD11b (Itgam) and F4/80 (Emr1), and express type I and type III collagen. (B): The relative percentage of collagen I expressing monocyte/macrophage cells in the wound increases dramatically at day 7 postwounding and slowly regresses toward baseline levels at subsequent time points (percentage relative to total Col I+ monocytes/macrophages). (C): Scatter plots representing correlation between collagen I expression and surface markers. There is heterogeneity in the expression of CD45 and CD11b among collagen I expressing hematopoietic cells.