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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Stem Cells. 2014 May;32(5):1347–1360. doi: 10.1002/stem.1648

Figure 6.

Figure 6

Two hematopoietic-derived fibrocyte populations are identified: GFP+/CD45+/CD11b+/Col I+ and GFP+/CD45−/CD11b−/Col I+. (A): Immunohistochemistry confirms the presence of a monocyte/macrophage subpopulation that deposits type I collagen. Purple: Collagen I, Blue: F4/80. Scale bar =20 μm. (B): Immunoblot demonstrating the recruitment of hematopoietic lineage (GFP+) Col I producing cells at day 7 postwounding, with an absence of this cell type in normal skin. Stromal cell (RFP+) Col I production also increases following wounding. (C): Flow cytometric analysis demonstrating that GFP+/CD45+/CD11b+/Col I+ cells are recruited to the wound, peak at day 7, and reduce in numbers by day 28 (percentage based on total GFP+ cells). (D): A second, non-monocyte hematopoietic cell subpopulation (GFP+/CD45−/CD11b−/Col I+) depositing type I collagen and demonstrating a similar recruitment pattern is also identified via flow cytometry (percentage based on total GFP+ cells). Abbreviations: GFP, green fluorescent protein; RFP, red fluorescent protein.

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