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. 2014 Jul 14;9(7):e101730. doi: 10.1371/journal.pone.0101730

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© 2014 Jin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The amino acid sequences were aligned by the Clustal W2 program, and the neighborjoining tree was drawn with TreeView. The corresponding GenBank and the melon genome (https://melonomics.net/) were noted in the phylogenetic tree and the accession number in the melon genome were CmCAD1 (MELO3C019548P1), CmCAD2 (MELO3C018492P1), CmACD3 (MELO3C003735P2), CmCAD4 (MELO3C005809P1) and CmCAD5 (MELO3C023272P1). The number for each interior branch was the percentage of bootstraps value (1000 replicates). Black circle denoted five CmCADs. CADs belong to the following plant species: Arabidopsis thaliana: AtCAD1 (AY288079), AtCAD2 (AY302077), AtCAD3 (AY302078), AtCAD4 (AY302081), AtCAD5 (AY302082), AtCAD6 (AY302075), AtCAD7 (AY302079), AtCAD8 (AY302080), and AtCAD9 (AY302076); Oryza sativa: OsCAD1 (AAN09864), OsCAD2 (DQ234272), OsCAD3 (AAP53892), OsCAD4 (BK003970), OsCAD5 (BK003971), OsCAD6 (CAD39907), OsCAD7 (CAE05206), OsCAD8A to D (BK003972), and OsCAD9 (AAN05338); Sorghum bicolor: SbCAD2 (Sb04g005950), SbCAD4-2 (Sb10g006300), SbCAD4-3 (Sb10g006290), SbCAD4-4 (Sb10g006280), SbCAD4-5 (Sb10g006270), SbCAD5 (Sb07g006090), SbCAD6 (Sb06g001430), SbCAD7 (Sb06g028240), SbCAD8-1 (Sb02g024220), SbCAD8-2 (Sb02g024210), and SbCAD8-4 (Sb02g024190); Triticum aestivum: TaCAD1 (GU563724), TaCAD2 (TC143210), TaCAD3 (TC143265), TaCAD4 (TC144004), TaCAD5 (TC149391), TaCAD6 (TC149393), TaCAD7 (TC170425), TaCAD8 (TC170426), TaCAD9 (TC170429), TaCAD10 (TC172690), and TaCAD11 (TC179401); Aralia cordata: AcCAD1 (D13991); Eucalyptus globulus: EgCAD1 (AF038561); Festuca arundinacea: FaCAD1a (AF188292); Lolium perenne: LpCAD1 (AF472591), LpCAD2 (AF472592), and LpCAD3 (AF010290); Medicago sativa: MsCAD1 (AF083333) and MsCAD2 (AF083332); Nicotiana tabacum: NtCAD1 (X62343) and NtCAD2 (X62344); Picea abies: PaCAD1 (X72675); Populus tremuloides: PtCAD1 (AF217957) and PtSAD (AF273256); Pinus taeda: PtaCAD1 (Z37992); Saccharum officinarum: SoCAD1 (AJ231135); Zea mays: ZmCAD1 (AJ005702) and ZmCAD2 (Y13733); Vitis vinifera: VvCAD (CBI34634.3), VvCAD6 (XP002269356.1), VvCAD9 (XP002279832.1). Cucumis sativus: CsCAD6 (XP004136373.1), CsCAD9 (XP004150677.1), CsCAD11 (XP004140716.1), CsCAD12 (XP004137094.1), CsCAD13 (XP004145884.1), CsCAD14 (XP004162965.1), Hordeum vulgare: HvCAD1A (BAJ84795.1), HvCAD1B (BAJ98188.1), HvCAD6 (BAK01962.1); Glycine max:GmCAD1 (XP003543132.1); Cicer arietinum: CaCAD1 (XP004485621.1); Gossypium hirsutum: GhCAD3 (ACQ59091.1); Ricinus communis: RcCAD (XP_002510582.1); Theobroma cacao: TcCAD9 (EOY15101.1); Fragaria vesca: FvCAD6 (XP004291336.1).