Table 1. Semi-quantitative PCR and real time PCR primers.
Gene | Primer | Sequence (5-3) |
CmCAD1 | CmCAD1-F | GAGACGCAAGAAGTATTG |
CmCAd1-R | ACTCAGGCATCTTACTAC | |
CmCAD2 | CmCAD2-F | CTTACACTTACGAACTCAG |
CmCAD2-R | CAACTTCCATCACTTCAC | |
CmCAD3 | CmCAD3-F | CCACAACACATCAACCAT |
CmCAD3-R | CATCCGCTAATCTTGCTTA | |
CmCAD4 | CmCAD4-F | CATTGTTGTTCACGAGAG |
CmCAD4-R | CCTATCACTCCAAGAGATT | |
CmCAD5 | CmCAD5-F | GCTGTTAAGATTGCTAAGG |
CmCAD5-R | GTAATCCATTGTCTCTGTTG | |
18srRNA | 18srRNA-F | AAACGGCTACCACATCCA |
18srRNA-R | CACCAGACTTGCCCTCCA |