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. 2014 Jul 14;9(7):e101627. doi: 10.1371/journal.pone.0101627

Figure 2. In vitro osteoinductivity of PLGA/HA composite scaffolds.

Figure 2

(A) MSC proliferation as measured by the Quant-iT PicoGreen Assay. (B) Mineralization as assessed using alizarin red staining of deposited calcium. Cell-based mineralization was quantified by subtracting scaffold-based mineralization and normalizing by cell count. (C) MSC OCN production induced by seeding on PLGA/HA scaffolds. Cell-secreted OCN as determined by ELISA and normalized by cell count. ND =  Not Detectable; N = 4; p<0.05 for + =  PLGA (same time point) and $ =  Same Experimental Group (Day 7).