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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Methods Enzymol. 2013;526:19–43. doi: 10.1016/B978-0-12-405883-5.00002-8

Figure 2.6.

Figure 2.6

FGF-2 stimulated H2O2 production in AHPs. After FGF-2 starvation, AHPs were loaded with 5 μM PF6-AM for 30 min, stimulated with H2O carrier (A) or 20 ng/mL FGF-2 (B), and then imaged. For DPI treatment, cells were preincubated in media containing 5 μM DPI before FGF-2 stimulation (C). For catalase addition, AHPs were transfected with either Catalase or control vector prior to FGF-2 stimulation (D). For Nox2 shRNA addition, AHPs were transfected with either Nox2-shRNA or control vector prior to treatment with FGF-2 (E).