Table 2.1.
Spectroscopic properties, localization, and example applications of selected boronate-based hydrogen peroxide probes
| Probe | Probe λabs/λem (nm) |
Product λabs/λem (nm) |
Intracellular localization |
Successful applications |
|---|---|---|---|---|
| Peroxyfluor 1 (PF1) | NA/NA | 494/521 | Nontargeted, diffuse |
Exogenous H2O2 detection in cells |
| Peroxyfluor 2 (PF2) | NA/NA | 475/511 | Nontargeted, diffuse |
Detection of H2O2 upon glucose treatment of hematopoietic stem cells in vivo in zebrafish |
| Peroxy green 1 (PG1) |
460/510 | 460/510 | Nontargeted, diffuse |
Detection of H2O2 in EGF signaling in A431 cells |
| Peroxy yellow 1 (PY1) |
494/558 | 519/548 | Nontargeted, diffuse |
Detection of H2O2 in EGF signaling in A431 cells, multiple colors |
| Peroxy orange-1 (PO1) |
507/574 | 540/565 | Nontargeted, diffuse |
Detection of H2O2 and HOCl during immune response in RAW264.7 macrophages; detection of H2O2 in FLT3/STAT5 signaling in leukemia cells |
| Peroxyfluor 6 acetoxymethyl ester (PF6-AM) |
460/530 | 492/517 | Cytosol | Detection of H2O2 in FGF-2 signaling in adult hippocampal progenitor cells, sodium delivery, or angiotensin II stimulation in the medullary thick ascending limb, and during chemotaxis of neutrophils |
| Mitochondria peroxy yellow 1 (MitoPY1) |
489,510/ 540 | 510/528 | Mitochondria | Detection of H2O2 upon paraquat treatment of HeLa cells, S100β, or APP treatment of DS neural progenitors, and sodium delivery in the medullary thick ascending limb |
| Nuclear peroxy emerald 1 (NucPE1) |
468,490/ 530 | 505/530 | Nucleus | Detection of H2O2 in vivo in a long-lived zebrafish model |