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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: J Mol Biol. 2014 May 2;426(13):2413–2421. doi: 10.1016/j.jmb.2014.04.023

Figure 1. Schematic of the experimental setup.

Figure 1

One end of a ssDNA construct is immobilized on a PEG-coated surface, and the other end is attached to a polystyrene bead confined in the optical trap (pink gray). A green excitation laser selectively illuminates molecules within a few hundred nanometers from the coverslip surface by total internal reflection (TIR).