Skip to main content
. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Oncogene. 2014 Jan 13;34(3):323–333. doi: 10.1038/onc.2013.553

Figure 4.

Figure 4

SUSD3 knockdown impairs cell-cell adhesion in MCF7 cells. (a) Cell-cell adhesion (dispase) assay. Number of cell fragments from control (siCTL) or SUSD3-deficient (siSUSD3 oligo 4) cultures counted with a dissecting scope after 30min incubation with 2.4 U/mL dispase. ***, p < 0.001. (b) Representative light microscopy image of cell fragments after the dispase assay (siCTL vs. siSUSD3). (c) Hanging drop aggregation assay. Number of fragments observed in siCTL vs. siSUSD3 oligo 4 prior to mechanical stress. **, p < 0.01. (d) Number of cells in clusters of 1-20, 21-100, >100 in hanging drops of non-triturated cells and (e) triturated cells. The percentage of cells in clusters is shown from experiments performed in triplicate.