Fig. 2.

Representative ethidium bromide-stained 2% agarose gel picture showing PCR amplification products of Cryptosporidium COWP gene sequence (≈ 550 bp) with primers Cry-9/Cry-15. Extracts of DNA were retrieved by the amended kit's protocol from 1 Cryptosporidium-positive fecal sample using 3 different approaches. M: GeneRuler™ 100 bp DNA marker; Lanes 1, 2, PCR products of 2 fecal aliquots subjected to direct DNA extraction; Lanes 3, 4, PCR products of 2 aliquots subjected to oocysts purification step prior to DNA extraction; Lane 5, A Cryptosporidium negative stool sample (extraction negative control). Lanes 6, 7, PCR products of two aliquots subjected to 6 freeze/thaw cycles prior to DNA extraction.