Table 1. Identification of tegumental associated proteins from FhTeg preparations by MSMS.

Peptide sequences were used to search against Genbank or a translated EST library for the identification of each protein. Sequences derived from MSMS analysis were interpreted either, automated or manually (where manually interpreted using Masslynx version 3.5 sequences are denoted by a *). Sequenced amino acids that match exactly with those found in the Genbank database or translated EST database are underlined.

Spot Number	Sequence	Accession Number	Identification	Species	MOTIFA)	% Contribution	ES ProductsB)	Tegument PreparationC)
1	-	-	Unidentified	Unidentified	-	-
2	1- TFWEFEEETPD*	Q7M4G0	FABP Type I	Fasciola hepatica	-	3.580	W
	1- NEIYGEYFSDPY*	XP_001149111	Heat Responsive Protein 12	Pan troglodytes	EM, TM(1)
	2- FQVACLPK*	XP_001149111
3	1- TLTFTFGEYLEEETDPGK	Q9UAS2	FABP Type I	F. hepatica	-	2.570	MRW
	2- CTVGDVK*	Q9UAS2
	3- TTFTFGEEFEEETPDG*	Q7M4G0
4	1- TTTFTFGEEFKDETFDNR*	Q7M4G1	FABP Type II	F. hepatica	-	7.012	MR	W1
	2- KMVITITVGDVK*
	3- WKLEHSENMDGVWK
	4- DFVGSWKLEHSENMDGVWK*
	5- PKPEFTFELEGNKMTIK*
5	1- PEVEFAKVDVDQNEEAAAK*	AAF14217	Thioredoxin	F. hepatica	-	1.407	R	W1
	2- YSVTAMPTFVFIK
	3- MPTFVFIKDGK
6	1- FVQESETSPVQIK	AAD30361	Cu/Zn Superoxide Dismutase	F. hepatica	-	0.657		W1
	2- AMVVHENEDDLGR
	3- SMSGSSGVQGTVK*
7	1- NKLLSPLGEGSPVAK	NP_082902	Methylmalonyl-CoA Epimerase, Mitochondrial Precursor	Mus Musculus	SP, EM	0.599
	2- DASGVLTELEAGASAPQ
	3- SGVLTELEETHSK
	4- EHGVTVAFVHFGNTK
	5- DASGVLTELEETP*
8	1- DQFTGAAPIFIK	AAX11353	Myoglobin 2	Paragonimus westermani	-	4.186	W
	2- LQGLTLFPVGQSEGIR
	3- LLKKTNPSVLEER
9	1- IIFELYTTLPK	AAT73777	TRIM5/Cyclophilin A Fusion Protein	Aotus trivirgatus	-	6.171	RW	W1-2
	2- HVVFGEVTPGYDVVK
	3- MEALGSASGQTSK	XP_721313	Cyclophilin Type Peptidyl-Prolyl Cis-Trans Isomerase	Candida albicans
	4- HVVFGEVTEGFDVVK	ATT91286	Putative Cyclophilin	Paxillus involutus
	5- IIPQMESQGGDFTGGATGTGGK	P0C1I2	Cyclophilin E	Rhizopus oryzae
	6- FFDITADGSALG*	XP_721313	Cyclophilin Type Peptidyl-Prolyl Cis-Trans Isomerase	Candida albicaximus
10	1- IATVTVGDVKAV*	Q9U1G6	FABP Type III	F. hepatica	-	4.863	MR	W1
11	1- GPGTAMPFALK	AAW26651	SJCHGC05973 Protein (GATase1_DJ-1)	Schistosoma japonicum	TM(1)	0.305		W1
	2- ITSYPGFEDK
	3- KITSYPGFEDK
	4- WTYCSGFGVVVDGNLVTSR
	5- IVAAICAAPIGLQPLAAALGR
12	1- IEIGLFGATVPK*	XP_001174161	Peptidylprolyl Isomerase B	P. Troglodytes	-	0.324	RW	W1-2
	2- DFMLQGGDFTDGDGTL*	O93826	Peptidyl-Prolyl Cis-Trans Isomerase B	Arthroderma benhamiae
13	1- AVPDKIDWR	AAR99518	Cathepsin L1 Protease	F. hepatica	SP	0.985	MRW	W1
	2- MCGIASLASLPMVAR*
14	1- ISMIEGAAMDLR*	ADP09370	Mu Class GST (GST 7, 26, 27, 28 or 47)	F. hepatica	-	3.681	MRW	W1
15	1- ISMIEGAAMDLR	ADP09370	Mu Class GST (GST 51)	F. hepatica	-	1.884	MRW	W1
	2- HGMLGSTPEER
	3- YLAPQCLEDFPK
16	1- AYGVLDEEQGNTYR*	CAA06158	Thiol-Specific Antioxidant Protein (TPX)	F. hepatica	TM(1)	1.604	RW	W1-3
17	1- VVIAYEPVWAIGTGK*	AAZ57433	Triose-Phosphate Isomerase	Orientobilharzia turkestanicum	-	1.617	RW	W1
	2- VASGAFTGEISPAMIR
	3- IFGESDELIGEK*
	4- IIYGGSVTAANCK	BAF62292	Triose Phosphate Isomerase	Schistosoma haematobium
18	1- VAVLGASGGIGQEGVLLLK	ABD77293	Mitochondrial Malate Dehydrogenase 2	Canis familiaris	EM, TM(1)	1.038	R	W1-2
	2- AQGSATLSMAYSGVR	AAT35230	Mitochondrial Malate Dehydrogenase	Clonorchis sinensis
19	1- ATEQVLAFTYK*	AAO85503	Aldolase	Pecten maximus	-	0.441	RW	W1-3
	2- PTFAQYLQPEQEAK	ABF55380	Fructose-1, 6-Bisphosphate Aldolase	S. japonicum
	3- LVDHLIQDGIIPGIK
20	1- VPTADVSVVDLTCR	AAG23287	Glyceraldehyde Phosphate Dehydrogenase	F. hepatica	EM, TM(1)	0.583	MR	W1 W3
	2- FPYDITIDQNK	AAA16243	Glyceraldehyde-3-Phosphate Dehydrogenase	S. japonicum
21	1- AVANVNSQIAPNLIK	Q27655/A53665	Enolase (2-Phosphoglycerate Dehydrogenase)/Phosphopyruvate Hydratase	F. hepatica	-	1.345	MRW	W1-2
22	1- QEYDESGPGIVHR*	AAW25537	SJCHGC06318 Protein Actin	S. japonicum	TM(2)	4.769	MRW	W1-3
	2- SYELPDGQVITIGNER	ABQ65136	Actin	Trichoderma atroviride	TM(1)
23	1- GTATLGGTCLNVGCIPSK*	YP_001202603	Dihydrolipoamide Dehydrogenase	Bradyrhizobium sp.	TM(1)	0.814
24	1- LIYSPTGALNTDTADIR	AAV59016	Leucyl Aminopeptidase	F. hepatica	-	0.448	W	W1
	2- NSVGADSYVADEILVAR
25	1- NQVAMNPNSKVFDAK	ABS52704	Heat Shock Protein 70	F. hepatica	-	2.386	W	W1-3
	2- TAGDTHLGSLTFDNR*
	3- TPSYVAFTDTER*

^A)Results from sequence analysis looking for signal peptides (SP), exportation to mitochondria (EM) and trans-membrane domains (TM). If TM was denoted the number of domains is reported in parentheses.

^B)If the identified proteins have been identified in a previous proteomics analysis of F. hepatica ES products they have been denoted with M, R or W for the studies of Morphew et al. (2007), Robinson et al. (2009) and Wilson et al. (2011) respectively.

^C)If proteins have been identified by Wilson et al. (2011) in tegumental preparations they have been denoted with W1, W2 or W3. W1- Cytosolic fraction; W2- Membrane associated; W3- Final pellet.