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. 2014 Mar 3;114(8):4564–4601. doi: 10.1021/cr400546e

Table 2. Intensity-Based, Small-Molecule Fluorescent Sensors for Zn2+.

  excitation
 emission
 brightnessb
      
sensor λfree (nm) εfreea λbound (nm) εbounda λfree (nm) φfree λbound (nm) φbound free bound DRc KD (M) ref
TSQ 380 ND 380 ND 495 ND 495 0.1 ND ND 100 ND (99)
Zinquin 370 ND 370 ND 490 ND 490 ND ND ND ND 2.0 × 10–10 (102)
3-Zn 343 7.6 343 6.8 450 0.038 450 0.88 0.2888 5.984 23 5.0 × 10–7 (109)
ZP1 515 67 507 78 531 0.38 527 0.87 25.46 67.86 3.1 7.0 × 10–10 (110)
ZP2 498 44 490 53 522 0.25 517 0.92 11 48.76 6 5.0 × 10–10 (111)
ZP3 502 75 492 85 521 0.15 516 0.92 11.25 78.2 6 7.0 × 10–10 (112)
ZP4 506 61 495 67 521 0.06 515 0.34 3.66 22.78 5 6.5 × 10–10 (113)
ZP5 504 83 495 91 520 0.29 517 0.48 24.07 43.68 1.6 5.0 × 10–10 (114)
ZP6 506 89 495 98 519 0.1 517 0.34 8.9 33.32 2.7 5.0 × 10–10 (114)
ZP7 505 68 495 77 521 0.04 517 0.05 2.72 3.85 0.4 5.0 × 10–10 (114)
ZP8 500 81 489 78 516 0.03 510 0.35 2.43 27.3 11 6.0 × 10–10 (115)
ZP9 505 51 494 44 526 0.02 521 0.41 1.02 18.04 12 6.9 × 10–7 (116)
ZP10 506 55 497 45 523 0.08 516 0.33 4.4 14.85 10 1.9 × 10–6 (116)
ZPF1 533 99 525 120 547 0.11 544 0.55 10.89 66 5 9.0 × 10–10 (112)
ZPCl1 534 97 527 120 550 0.22 549 0.5 21.34 60 1.8 1.1 × 10–9 (112)
ZPBr1 534 45 528 86 549 0.25 547 0.36 11.25 30.96 1.7 9.0 × 10–10 (112)
ZPF3 520 87 510 93 537 0.14 533 0.6 12.18 55.8 3.6 8.0 × 10–10 (112)
Me2ZP1 515 74 505 80.6 528 0.18 524 0.61 13.32 49.166 4 3.3 × 10–9 (117)
Me4ZP1 516 56 506 47.4 529 0.17 525 0.35 9.52 16.59 2 6.0 × 10–7 (117)
ZPP1 505 ND 500 ND 532 0.052 523 0.7 ND ND 13 5.1 × 10–9 (118)
DA-ZP1-TPP 510 ND 510 ND 529 0.001 529 0.75 ND ND 12 6.0 × 10–10 (68)
ZS1 510 83.9 501 75.2 531 0.5 526 0.7 41.95 52.64 1.4 ND (119)
ZS2 499 66.9 489 67.6 523 0.39 516 0.69 26.091 46.644 2 ND (119)
ZS3 500 86.9 ND ND 525 0.71 525 NA 61.699 ND 1 ND (119)
ZS4 507 81.1 495 ND 522 0.12 520 0.5 9.732 ND 4.5 ND (119)
ZS5 497 33 490 42 522 0.36 517 0.8 11.88 33.6 4.6 1.5 × 10–6 (120)
ZS6 515 ND 505 ND 533 0.44 527 0.64 ND ND 3.3 ND (120)
ZS7 500 62 490 66 524 0.25 518 0.79 15.5 52.14 2.7 3.7 × 10–6 (120)
ZSF6 532 63 522 70 549 0.19 545 0.63 11.97 44.1 2 4.6 × 10–6 (120)
ZSF7 521 62 511 70 535 0.24 527 0.68 14.88 47.6 2.5 5.0 × 10–6 (120)
QZ1 505 68.9 498 69.8 524 0.024 524 0.78 1.6536 54.444 42 3.3 × 10–5 (121)
QZ2 499 37.2 489 33.6 520 0.005 518 0.7 0.186 23.52 150 4.1 × 10–5 (121)
QZ2E 499 27.2 496 16 519 0.004 514 0.73 0.1088 11.68 120 1.8 × 10–3 (122)
QZ2A 498 64.1 492 40 515 0.012 515 0.51 0.7692 20.4 30 1.3 × 10–4 (122)
FluoZin-1 496 ND 496 ND 515 ND 515 ND ND ND 200 7.8 × 10–6 (123)
FluoZin-2 495 ND 495 ND 525 ND 525 ND ND ND 12 2.1 × 10–6 (123)
FluoZin-3 495 ND 495 ND 516 ND 516 ND ND ND 200 1.5 × 10–8 (123)
ZnAF-12 492 74 492 63 514 0.02 514 0.23 1.48 14.49 17 7.8 × 10–10 (124)
ZnAF-23 492 78 492 76 514 0.02 514 0.32 1.56 24.32 51 2.7 × 10–9 (124)
ZNAF-1F 489 77 492 70 515 0.004 515 0.17 0.308 11.9 69 2.2 × 10–9 (125)
ZnAF-2F 490 74 492 73 515 0.006 515 0.24 0.444 17.52 60 5.5 × 10–9 (125)
ZnAF-2M 490 53 492 52 515 0.03 515 0.27 1.59 14.04 6.8 3.8 × 10–8 (126)
ZnAF-2MM 490 111 493 88 515 0.01 515 0.1 1.11 8.8 12.3 3.9 × 10–6 (126)
ZnAF-3 490 71 493 62 515 0.03 515 0.38 2.13 23.56 10.4 7.9 × 10–7 (126)
ZnAF-4 490 68 492 64 515 0.01 515 0.22 0.68 14.08 16.3 2.5 × 10–5 (126)
ZnAF-5 490 64 492 43 515 0.004 515 0.21 0.256 9.03 34.3 6.0 × 10–4 (126)
Newport Green DCF 505 ND 505 ND 535 ND 535 ND ND ND 5 4.0 × 10–5 (127)
Newport Green PDX 495 ND 495 ND 520 ND 520 ND ND ND ND 3.0 × 10–4 (123)
ZIMIR 493 73 493 73 515 0.0032 515 0.225 0.2336 16.425 70 4.5 × 10–6 (69a)
ZnAB 499 ND 499 ND 509 0.003 509 0.058 ND ND ND ND (128)
BDA 491 19.5 491 18 509 0.077 509 0.857 1.5015 15.426 10.5 1.0 × 10–9 (129)
WZS 499 17.1 499 17.1 550 0.03 550 0.19 0.513 3.249 6 6.2 × 10–10 (130)
RhodZin-3 550 ND 550 ND 575 ND 575 ND ND ND 75 6.5 × 10–5 (67a)
RA1 535 45 535 1.3 561 0.7 561 0.78 31.5 1.014 ND 1.3 × 10–6 (131)
ZRL1 569 ND 569 20.8 595 <0.001 595 0.22 ND 4.576 220 7.3 × 10–5 (132)
Rhod-5f 571 ND 571 ND 594 0.28 594 0.13 ND ND 11.6 4.1 × 10–6 (133)
ZBR1 514 19.3 525 26.4 625 0.067 628 0.41 1.2931 10.824 8.4 6.9 × 10–10 (70)
ZBR2 550 16.9 530 25.6 630 0.069 630 0.22 1.1661 5.632 ND 7.0 × 10–10 (70)
ZBR3 530 13.3 535 19.3 623 0.342 628 0.6 4.5486 11.58 ND 1.0 × 10–12 (70)
DPA-CY 606 150 606 190 800 0.02 800 0.41 3 77.9 20 6.3 × 10–8 (70)
SiR-Zn 650 98 651 110 665 0.009 665 0.12 0.882 13.2 15 1.4 × 10–9 (134)
a

Molar extinction coefficients given as ε/103 M–1 cm–1.

b

Brightness is defined as the product of the molar extinction coefficient and the quantum yield (ε × φ).

c

There is no systematic way to present dynamic range (DR), so we encourage readers to refer to the original publications for more details about this value. For intensity-based probes, this number is generally the maximum fold change in fluorescence intensity upon Zn2+ binding. ND, not determined.