Table 6. Genetically Encoded Cu+ Sensors.
| FP |
excitation |
emission |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| name | donor | acceptor | λdonor (nm) | εdonora | λacceptor (nm) | εacceptora | λdonor (nm) | φdonor | λacceptor (nm) | φacceptor | DR (Rmax/Rmin) | KD (M) | ref |
| Amt1-FRET | ECFP | EYFP | 439 | 32.5 | 514 | 83.4 | 476 | 0.60 | 527 | 0.61 | ND | 2.50 × 10–18 | (178) |
| Ace1-FRET | ECFP | EYFP | 439 | 32.5 | 514 | 83.4 | 476 | 0.60 | 527 | 0.61 | ND | 4.70 × 10–18 | (166b) |
| Mac1-FRET | ECFP | EYFP | 439 | 32.5 | 514 | 83.4 | 476 | 0.60 | 527 | 0.61 | ND | 9.70 × 10–20 | (166b) |
| eCALWY-C2M/C3M | Cerulean | Citrine | 433 | 43.0 | 516 | 77.0 | 475 | 0.62 | 529 | 0.76 | ND | <1 × 10–17 | (182) |
| YAG(n), n = 0 | EYFP | ND | 514 | 83.4 | ND | ND | 527 | 0.61 | ND | ND | ND | 8.20 × 10–18 | (181) |
| YAG(n), n = 1 | EYFP | ND | 514 | 83.4 | ND | ND | 527 | 0.61 | ND | ND | ND | 2.00 × 10–18 | (181) |
| YAG(n), n = 2 | EYFP | ND | 514 | 83.4 | ND | ND | 527 | 0.61 | ND | ND | ND | 1.20 × 10–18 | (181) |
| YAG(n), n = 3 | EYFP | ND | 514 | 83.4 | ND | ND | 527 | 0.61 | ND | ND | ND | 4.60 × 10–19 | (181) |
| YAG(n), n = 4 | EYFP | ND | 514 | 83.4 | ND | ND | 527 | 0.61 | ND | ND | ND | 3.30 × 10–19 | (181) |
| EGFP-145Amt1 | EGFP | ND | 484 | 32.5 | ND | ND | 507 | 0.60 | ND | ND | ND | 4.60 × 10–19 | (179) |
a
Molar extinction coefficients given as ε/103 M–1 cm–1. ND, not determined.