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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Vaccine. 2011 Dec 23;30(8):1492–1501. doi: 10.1016/j.vaccine.2011.12.064

Fig. 7.

Fig. 7

Ad5-Clostridium difficile-TA vaccination completely protects mice from lethal TA challenge. (A) WT BALB/c mice were IM injected (1010 vp/mouse) with Ad5-Null (n = 5) or Ad5-C. difficile-TA (n = 5). At 14 dpi mice were IP challenged with 300 ng (6 × LD50 ) of purified toxin A, purchased from Calbiochem. Kaplan–Meyer survival curves are shown. Curves were compared by log-rank analysis and were found to be significantly (p < 0.05) different. (B) WT BALB/c mice were left uninjected (naïve, n = 11) or were IM injected (1010 vp/mouse) with Ad5-Null (n = 8) or Ad5-C. difficile-TA (n = 7). At 14 dpi mice were IP challenged with 300 ng (6 × LD50 ) of purified toxin A, purchased from List Biological Laboratories Inc. Kaplan–Meyer survival curves are shown. Curves were pair-wise compared by log-rank analysis and the following results were obtained: naïve versus Ad5-TA curve were significantly (p = 0.0043) different, Ad5-Null versus Ad5-TA curves were significantly (p = 0.036) different, however naïve versus Ad5-Null curves were not significantly different (p = 0.486).