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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Vaccine. 2011 Dec 23;30(8):1492–1501. doi: 10.1016/j.vaccine.2011.12.064

Table 1.

C. difficile toxin A specific major T cell epitopes.a

400+ SFCs 150–400 SFCs 100–150 SFCs 50–100 SFCs
Number of responders 2 3 4 2
Peptides #13 VNGSRYYFDTDTAIA #9 NEKYYFNPN-NAIAAV #27 YFNTNTA-IASTGYTI
#30 IGVFKGP-NGFEYFAP		 #66 QIGVFKG-PDGFEYFA
#63 YYFNTNTSIASTGYT #51 QTIDGKKYYFNT-NTF #36 KYYFNPN-NAIAAIHL #74 NKN-FYFRNGLPQIGV
#55 VFKGP-NGFEYFAPAN #37 AAIHLCTINND-KYYF
a

C. difficile toxin A specific major T cell epitopes. Splenocytes derived from Ad5-TA vaccinated BALB/c mice were stimulated with 15-mer library spanning the C. difficile TA region, encoded by Ad5-TA vaccine, followed by ELISPOT assay. Peptides with most dramatic SFC (spot forming cells) count are presented (most immunogenic epitopes). Note that H2Kd (9-mers) epitope prediction (http://www.syfpeithi.de/scripts/MHCServer.dll/) yields derivatives of peptides #13 and #63 as 2 top hits (highlighted in bold and underlined).