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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Biol Chem. 2005 Jul 20;280(37):32230–32237. doi: 10.1074/jbc.M504553200

FIGURE 2. siRNA knockdown of TRPC4 protein expression.

FIGURE 2

First lane, protein molecular mass markers (M; in kDa); second lane, non-transfected cells (control (Con)); third lane, cells transfected with siCONTROL (siCON; non-targeting siRNA); fourth lane, cells transfected with siRNA2; fifth lane, cells transfected with siRNA3. In the sixth lane, anti-TRPC4 antibody pre-absorbed to epitope recognized by this antibody did not detect the TRPC4 band. Immunoblot analyses showed that two of the four synthesized TRPC4 siRNAs (i.e. siRNA2 and siRNA3) effectively decreased TRPC4 protein expression relative to their controls. Preimmu., preimmune.