FIGURE 5. Identification of SOC currents by passive store depletion in non-transfected HCEC.

A, experimental design; B, voltage pulse protocol; C, depolarization pulse-induced currents after establishing the whole cell configuration (0 mm) (upper panel) and currents induced in the same cell after CPA-induced store depletion (10 min) in the presence of 5 mm Ca²⁺ (lower panel); D, current-voltage relationship (I-V plot). After breaking into the whole cell configuration (0 mm) (○), no SOC currents were observed; 10 min later, a significant increase in CPA-induced SOC currents could be observed with 5 mm Ca²⁺ as charge carrier (n = 5) (●). HP, holding potential.