FIGURE 7. EGF-induced SOC activation is dependent on TRPC4 protein expression.
A, currents after establishing the whole cell configuration (upper panel) and EGF (20 ng/ml)-induced increases in SOC inward currents (lower panel). B, I-V plot. After breaking into the whole cell configuration (○), a significant increase in EGF-induced SOC currents was observed in non-transfected HCEC (20 ng/ml EGF; n = 4; p < 0.005). C, no significant increases in currents observed in either the absence (upper panel) or presence (lower panel) of 20 ng/ml EGF in TRPC4 siRNA-transfected HCEC. D, I-V plot revealing that EGF-induced increases in SOC currents were suppressed in TRPC4 siRNA-transfected HCEC (n = 4; p > 0.64).