Fig. 1.

Expression of ${\mathrm{HCO}}_3^{-}$-activated soluble adenylyl cyclase (sAC) in corneal endothelium. A: RT-PCR for sAC. M, markers; BCEC, cultured bovine corneal endothelial cells; NC, negative control (no RT). B and C: indirect immunofluorescence for sAC in cultured BCEC. B: primary sAC antibody. C: no sAC antibody. D: ${\mathrm{HCO}}_3^{-}$-activated intracellular cAMP concentration ([cAMP]) in BCEC. Cells were incubated in ${\mathrm{HCO}}_3^{-}$-free DMEM at 37°C for 3 h and then for 30 min in 0.3 mM α,β-methyleneadenosine 5′-diphosphate (AMP-CP) at different [${\mathrm{HCO}}_3^{-}$], with (▲) or without (■) 50 μM rolipram (Rol). Error bars indicate ±SE (n = 6).