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. 2014 Jul 14;9(7):e101991. doi: 10.1371/journal.pone.0101991

Figure 5. SR induces DNA damage in REH cells.

Figure 5

Cells were treated with increasing concentrations of, or for increasing time intervals with, SR and immediately assayed for DNA breakage with FHA. A) DNA single strand breakage induced by 3 h SR treatment in REH cells. B) DNA single strand breakage induced by 30 µM SR in REH cells as a function of incubation time. Data are presented as mean ± SEM of three different experiments. C) and D) Representative, digitally pseudocolored (ICA look up table of the Image J software) micrographs of FHA-processed control (C) or 30 µM SR-treated (D) REH cells are also shown: note the wide halos in D as compared to C. Also shown (panel A, inset) the extent of DNA double strand breakage caused by SR (30 µM for 3 h) or etoposide (1 µg/mL for 3 h) in REH cells. Data are expressed as NSF (nuclear diffusion factor), which represents the ratio between the total area of the halo and nucleus and that of the nucleus.