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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Hypertension. 2011 Aug 1;58(3):431–438. doi: 10.1161/HYPERTENSIONAHA.111.172338

Figure 2.

Figure 2

Effect of Pin1 small interfering RNA (siRNA) on endothelial NO synthase (eNOS) Ser116 phosphorylation. Rat aortic endothelial cells were transfected with Pin1 or scrambled siRNA. Representative Western blots showing (A) Pin1 expression, (B) eNOS Ser116 phosphorylation, (C) eNOS Ser1177 phosphorylation, and (D) eNOS Thr495 phosphorylation and densitometry for ratio of Pin1:actin or eNOS phosphorylation:eNOS as a percentage of control. Actin was used as a loading control. Results are expressed as mean±SEM (n>3 independent experiments). *P<0.05 vs control.