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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Hypertension. 2011 Aug 1;58(3):431–438. doi: 10.1161/HYPERTENSIONAHA.111.172338

Figure 5.

Figure 5

Effect of chronic Pin1 inhibition on vascular endothelial NO synthase (eNOS) function and systolic blood pressure in mice. Control mice were treated daily for 2 weeks with either juglone (1 mg/kg, IP) or vehicle. A, Representative Western blot showing aortic eNOS Ser116 phosphorylation. B, Aortic NO production as measured by Nω-nitro-l-arginine (LNNA)–sensitive 4-amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM) fluorescence. C, Aortic relaxation responses to acetylcholine after contraction with phenylephrine in the absence and presence of NO synthase (NOS) inhibition (LNNA). Relaxation to 100% represents a full return to a passive tension of 0.75 g. D, Systolic blood pressure at baseline, day 7, and day 14 of juglone or vehicle treatment. Results are expressed as mean±SEM (n>5 mice for each group). *P<0.05 vs control, +P<0.05 vs day 7 blood pressure.