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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Exp Biol Med (Maywood). 2014 Mar 5;239(5):581–594. doi: 10.1177/1535370214522183

Figure 5.

Figure 5

The effect of PI3K/Akt pathway on DAF expression was not mediated by NO. The HeLa cells were transiently transfected with PTEN-specific siRNA or a scrambled siRNA (control) for 24 hours and then treated with either nitric oxide synthase inhibitor L-NAME or NO donor DETANONOate for 24 hours followed by cell lysis for real time qPCR and western blot respectively. (a) Fold change in the expression of DAF mRNA in HeLa cells. * p < 0.0001. (b) Western blot for total PDK1, total Akt and their activated forms, pPDK1, pAkt(ser473) and pAkt(Thr308) in Ishikawa cells. (c) Western blot density analysis for total PDK1, total Akt and their activated forms, pPDK1, pAkt(ser473) and pAkt(Thr308) in Ishikawa cells. * p < 0.05 versus control, ** p < 0.001 versus control, † p < 0.05 versus control, †† p < 0.05 versus control, ‡ p < 0.05 versus control. Data are representative images or expressed as mean values ± SEM of each group from three separate experiments.