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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Alcohol. 2014 Jun 7;48(5):483–492. doi: 10.1016/j.alcohol.2014.06.001

Figure 2. Representative images of cell culture conditions.

Figure 2

Neural progenitor cells were collected from E15–E17 tissue without exposure to alcohol and expanded as adherent monolayer cultures in the presence of growth factors (EGF, FGF-2) for immunohistochemistry. Fig. 2A is a 20X representative image of the proliferating NPC culture conditions; Nestin, which marks neural progenitor cells, is in green with a DAPI nuclear stain in blue. Fig. 2B is a 20X representative image of the differentiated NPC culture conditions; GFAP, which marks astrocytes, is in green with a DAPI nuclear stain in blue and the immature neuronal marker doublecortin (DCX) in red.