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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Cell Biochem. 2013 Apr;114(4):782–795. doi: 10.1002/jcb.24416

Figure 4.

Figure 4

FFS-induced changes in LIMK phosphorylation

Confluent monolayers of BAECs were exposed to 15dynes/cm2 FSS for 15 and 30 mins and labeled with antibodies against pLIMK1/2 (threonine-508/505) [A] or pLIMK1L (serine-323) [B]. Image analysis was performed as described in the methods. The images shown are representative of at least 4 replicates. [A]. Top panel, uninhibited BAECs. Bottom panel, SP600125-treated BAECs. [B]. Uninhibited BAECs. Scale bars = 10μm.