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. Author manuscript; available in PMC: 2015 Jul 25.
Published in final edited form as: Neuroscience. 2014 May 15;273:128–140. doi: 10.1016/j.neuroscience.2014.05.016

Figure 6.

Figure 6

Crush of terminal muscle branch results in continued disruption of nerve diffusion. Cohorts of N=2 animals in each group received a ligation of the parent femoral nerve and during the same surgery the terminal muscle branch was crushed; a control group received a sham crush. At 0, 2, or 10 days after this initial surgery Alexa-647-labeled dextran was applied distal to the nerve crush site. Eighteen hours after dextran application the nerves were harvested and processed as flattened whole mounts. The white triangle represents the nerve crush site. A nerve crush applied even 10 days earlier still blocks diffusion of the labeled dextran.