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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2013 Nov 29;98(4):1737–1747. doi: 10.1007/s00253-013-5368-x

Fig. 5.

Fig. 5

Affinity purification and Western analysis of Rfa3 and Rfa8 in Halobacterium sp. NRC-1 and derivative strains. Total protein samples from cell lysates and fractions binding to a DNA-cellulose column were electrophoresed on a 12% polyacrylamide-SDS gel, and stained with Coomassie blue, or transferred to PVDF membrane, and probed with rabbit Rfa3 antibody followed by a secondary goat anti-rabbit antibody-alkaline phosphatase conjugate and substrate. Panel A contains Halobacterium sp. NRC-1, panel B contains Halobacterium sp. LH5, panel C contains Halobacterium sp. LH7a, and panel D contains Halobacterium sp. NRC-1 (pDRK1). Lanes labeled 1 contain total cell lysate and lanes labeled 2 contain peak bound fraction from a DNA-cellulose column, Coomassie stained, and lanes labeled 3 display results of Western blots.