Table 1.
Gene | Accession number | Primer sequence | Amplicon size (bp) |
---|---|---|---|
BCLXL | NM_001082135.1 | 5′aggagatggaggtattggtgagtc3′ | 122 |
5′gttgccgtagagttccacaaac3′ | |||
HDAC1 | XM_002720715.1 | 5′gctgaggagatgaccaagtacc3′ | 173 |
5′acagagccaccagtagacag3′ | |||
H2A | AF030235.1 | 5′caaggcagtgtctcgctcacag3′ | 187 |
5′gagatccttggaagcgttacctgc3′ | |||
HPRT1 | EF062857.1 | 5′ataagttctttgctgacctgctg3′ | 134 |
5′tacttttatgtcccctgttgactg3′ | |||
KLF4 a | 5′ctcaaggcacacctgcgaac3′ | 193 | |
5′tcttcatgtgtaaggcgaggt3′ | |||
NANOG a | 5′cagccctgattcttctaccagtcc3′ | 196 | |
5′ggagagttcttgcatctgctggag3′ | |||
OCT4 | EF062856.1 | 5′ggtgttcagccaaaccaccatctg3′ | 352 |
5′ttgggaacagtcactgcttgatcg3′ | |||
SOX2 a | 5′cgggtgctccttcatgtgcag3′ | 120 | |
5′cacaactcggagatcagcaagc3′ | |||
YWHAZ | ENSOCUG00000000734 | 5′ggtctggcccttaacttctctgtgttcta3′ | 142 |
5′gcgtgctgtctttgtatgattcttcactt3′ |
aPrimers designed from conserved regions of human, murine and bovine sequences