Comparative analyses of LDH isoenzyme expression levels in synaptosomal fractions from the neocortex and striatum A) A representative immunoblot showing LDH isoenzymes in the neocortical and striatal synaptosomal fractions. An isoform indifferent antibody was utilized to detect the total level of LDH and to serve as an internal loading control. B) The densitometry evaluated level of LDH-A and LDH-B proteins, as determined by Western blot, are shown. C) Bar graphs show relative LDH isoenzymes level as measured by ELISA in neocortical and striatal synaptosomes. * and # symbols indicate P ≤ 0.05 for Mann-Whitney U-test between strepsirrhines and haplorhines for fractional LDH-A and LDH-B levels, respectively. ^ and & indicate P ≤ 0.05 for Kruskal–Wallis test among strepsirrhines, “monkeys”, and chimpanzees and humans for LDH-A and LDH-B, respectively.