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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Nat Biotechnol. 2013 Nov 17;32(1):76–83. doi: 10.1038/nbt.2747

Figure 2. Acinar cells are the primary source of new beta-like cells in diabetic mice treated with EGF and CNTF.

Figure 2

(A) Scheme of the experimental design for cell lineage tracing. (B) RipCreERTR26LacZ mice were treated or not with ALX and implanted with pumps as indicated (see Figure 1 for details). At 7 days after pump implantation, the pancreas of each mouse was stained with XGal, with antibodies specific for PDX1 and amylase (AMY). DNA was stained with Hoechst 33342. (C) Quantification of labeled cells based on data shown in (B) (*: p<0.01; n=13). (D) ElaCreERTR26LacZ mice were treated or not with ALX and implanted with pumps as indicated (see Figure 1 for details). At 7 days after pump implantation, the pancreas of each mouse was stained with XGal, with anti-insulin (INS), anti-amylase (AMY) and Hoechst 33342. (E) Quantification of labeled cells based on data shown in (D) (*: p<0.01; n=13). Overall Xgal labeling, representing the recombination efficiency, was >80% in RipCreERTR26LacZ mice and 45% in ElaCreERTR26LacZ mice.