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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Methods Cell Biol. 2011;104:195–208. doi: 10.1016/B978-0-12-374814-0.00011-2

Figure 5.

Figure 5

Tamoxifen-dependent recombination. Individual vc2 green-to-red reporter embryos injected with mRNA for PhiC31o (A,D,G) or PhiC31o-ERT2 (B,C,E,F,H,I) are shown. With addition of DMSO alone (B,E,H) no DsRed expression is observed, while addition of 300 nM 4-hydroxytamoxifen (C,F,I) induces recombination and red fluorescence (I), although to a much lesser degree than non-chimeric PhiC31o integrase (G).