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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Biochemistry. 2008 Aug 26;47(38):9987–9999. doi: 10.1021/bi800746p

Figure 4.

Figure 4

Agarose gel electrophoresis showing variability in products generated after isothermal amplification for 2 h at 55 °C in the presence of 100 nM template but no trigger. (A) Comparison of products formed using different template sequences: lane 1, 10 bp ladder; lanes 2 and 3, template Ran2; lanes 4 and 5, template Ran15; lanes 6 and 7, template Ran17; lanes 8 and 9, template Ran21; lanes 10 and 11, template Ran24; lane 12, 100 bp ladder. Replicates represent samples from two separate runs per template, performed on the same day. (B) Comparison of products formed in replicate runs using the same template sequences (Ran24): lane 1, 10 bp ladder; lanes 2–6, runs 1–5 on day 1; lanes 7–11, runs 1–5 on day 2; lane 12, 100 bp ladder.