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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Biochemistry. 2008 Aug 26;47(38):9987–9999. doi: 10.1021/bi800746p

Figure 6.

Figure 6

LC–ESI MS analysis of products from nonspecific background amplification, with an emphasis on product peaks appearing after short amplification times. LC traces (left) and corresponding mass spectra (right) for EXPARs using template sequence (A) StanA [trigger m/z 1573 (2−) and 1048 (3−)] and (B) Ran 2 [trigger m/z 1496 (2−) and 997 (3−)]. Positive reaction mixtures contained 1 pM trigger, enzymes at standard concentrations, and 100 nM template. Negative reaction mixtures contained no trigger but did contain enzymes at standard concentrations and 100 nM template. Blank reaction mixtures contained only 100 nM template.