Table 1.
Selected EXPAR Trigger and Template Sequencesa
| Name | Trigger | Template |
|---|---|---|
| Stan A | 5′-CAGTCGTAGG-3′ | 5′-CCTACGACTGAACAGACTCTCCTACGACTG-3′-NH2 |
| SNP 2 | 5′-CCGTCGTAGG-3′ | 5′-CCTACGACGGAACAGACTCTCCTACGACGG-3′-NH2 |
| SNP 8 | 5′-CAGTCGTTGG-3′ | 5′-CCAACGACTGAACAGACTCTCCAACGACTG-3′-NH2 |
| SNP 9 | 5′-CAGTCGTACG-3′ | 5′-CGTACGACTGAACAGACTCTCGTACGACTG-3′-NH2 |
| SNP 10 | 5′-CAGTCGTAGC-3′ | 5′-GCTACGACTGAACAGACTCTGCTACGACTG-3′-NH2 |
| Ran 2 | 5′-CTATTTCCCC-3′ | 5′-GGGGAAATAGGTGAGACTCTGGGGAAATAG-3′-NH2 |
| Ran 15b | 5′-TTATCACACG-3′ | 5′-CGTGTGATAAGAGCGACTCTCGTGTGATAA-3′-NH2 |
| Ran 17 | 5′-CCGCGCCCCG-3′ | 5′-CGGGGCGCGGTGCGGACTCTCGGGGCGCGG-3′-NH2 |
| Ran 21c | 5′-GGTTAAATCG-3′ | 5′-CGATTTAACCTGATGACTCTCGATTTAACC-3′-NH2 |
| Ran 23 | 5′-CAGACGATGT-3′ | 5′-ACATCGTCTGCGGCGACTCTACATCGTCTG-3′-NH2 |
| Ran 24 | 5′-GCTGCACGCT-3′ | 5′-AGCGTGCAGCGTGGGACTCTAGCGTGCAGC-3′-NH2 |
| X′ rY′ | N/A | 5′-CGTGTGATAAGAGCGACTCTCGATTTAACC-3′-NH2 |
| X′ sX′ | N/A | 5′-CGTGTGATAAATGACATATTCGTGTGATAA-3′-NH2 |
a
Template sequence design: underlined portions denote the trigger complement, bold portions the nicking enzyme recognition sequence, and italic portions the four-base spacer sequence after which the nicking enzyme cuts the top strand.
b
X′rX′ is equal to Ran 15.
c
Y′rY′ is equal to Ran 21.