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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Microbiol. 2014 Feb 8;91(5):976–995. doi: 10.1111/mmi.12509

Fig. 3. The emrA1 mutant of F. tularensis is highly sensitive to oxidative stress.

Fig. 3

(A) F. tularensis (Ft) LVS, emrA1 and emrA1+ pemrA1 transcomplemented strains were grown in MH-broth containing 1000 μM of H2O2. The cultures were grown for 24 hrs and OD600 readings were recorded every 4 hrs.

(B) The bacterial killing assay. The bacterial cultures were exposed to 1000 μM of H2O2, 1 mM Pyrogallol or 1mM Paraquat for 1 and 3 hrs (n=3 biological replicates). The cultures were diluted 10-fold and plated on MH-chocolate agar plates for bacterial enumeration. The results are expressed as Log10 CFU/ml. The data are representative of three independent experiments conducted with identical results. The data were analyzed by ANOVA with a Tukey-Kramer post-test, and a cut-off p value of 0.05 or less was considered significant. Comparisons are shown with Ft LVS. **, p<0.01; ***, p<0.001.

(D) Disc diffusion assay with superoxide generating compound pyrogallol (Concentration = 50μg/disc).