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. Author manuscript; available in PMC: 2014 Nov 13.
Published in final edited form as: Nat Commun. 2014 May 13;5:3734. doi: 10.1038/ncomms4734

Fig. 4.

Fig. 4

Rab11 protein levels in ectodermal cells with manipulated PCP signaling. Embryos were animally injected with Vangl2 MO (20 ng) or Diversin MO (30 ng) (a) or Rab11 (100 pg), Shroom (0.3 ng) and Vangl2 (1 ng) RNAs (b) and the levels of Rab11 in isolated neural (dorsal, D) and non-neural (ventral, V) explants (stage 14, a) or ectoderm explants made at stage 12 (b) were determined by immunoblot analysis. WE, whole embryo. α-Tubulin is a loading control. The quality of dissection for neural and non-neural explants was assessed by the pan-neural marker Sox3. a, Although Rab11 levels are higher in neuroectoderm, there was no change after Diversin and Vangl2 depletion. b, Rab11 levels were not affected in ectoderm explants after Shroom and/or Vangl2 RNA injection.