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. 2014 Jul 10;12:193. doi: 10.1186/1479-5876-12-193

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Copyright © 2014 Xu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Chemotaxis of CXCL5 on LCCs. A: Transfection efficiency with the ratio of Lipofectame 2000 and siRNA at 1:20, including bright field (A1), FAM (A2), and merged (A3) (×200); B: Inhibitory efficiency of three siRNAs of CXCL5: relative mRNA levels of CXCL5 48hs after siRNA transfection measured by RT-PCR (B1) and protein levels of CXCL5 72hs after siRNA transfection measured by ELISA (B2); C: Effect of CXCL5 on LCC migration and invasion measured by transwell assay (×200); D: Average cell numbers of migration and invasion in three identical experiments (*P < 0.05, **P < 0.01, ***P < 0.001).