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. 2014 Jul 14;9(7):e102407. doi: 10.1371/journal.pone.0102407

Figure 5. Ann5 overexpression did not influence pollen germination and tube growth in response to LatB.

Figure 5

(A) Representative images of pollen grains and tubes from the Lat52-GFP, Lat52-Ann5-GFP 1 and 3 and Ann5Pro-Ann5 1 homozygous lines growing on germination medium containing 1 or 2 nM LatB for 3 h. Bar  = 50 µm. (B) Pollen germination ratios of the Lat52-GFP, Lat52-Ann5-GFP 1 and 3 and Ann5Pro-Ann5 1 homozygous lines after the pollen had germinated for 3 h in the presence of 1 or 2 nM LatB. Values represent the means ± SD. Eight hundred pollen grains and tubes were counted. (C) Pollen tube lengths of the Lat52-GFP, Lat52-Ann5-GFP 1 and 3 and Ann5Pro-Ann5 1 homozygous lines after the pollen germinated normally for 2 h and then for 2 h in the presence of 2.5 or 5 nM LatB. Values represent the means ± SD. Five hundred pollen tubes were measured.