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. 2014 Jul 14;9(7):e102407. doi: 10.1371/journal.pone.0102407

Figure 8. Ann5Pro-G26 and Ann5Pro-G257 only partially rescued the aborted pollen genotype in Lat52-Ann5UTRi plants.

Figure 8

(A) The expression level of the Ann5 transcript in Lat52-Ann5UTRi and individual complementation plants. Total RNA was isolated from the open flowers and used for RT-PCR. EF4A was used as the control. (B) Quantification of the sterile pollen grains from Lat52-Ann5UTRi and the individual complementation plants. Values represent the means ± SD. At least 500 pollen grains were counted. **P<0.01 by Student's t test. (C) Recovery analysis of the pollen lethality phenotype. Ann5Pro-Ann5 fully rescued the sterile pollen phenotype in the Lat52-Ann5UTRi lines. Ann5Pro-G26 and Ann5Pro-G257 only partially rescued the aborted pollen genotype. Bright-field images were obtained with a microscope 1 h after germination. Red stars indicate the aborted pollen grains. Bars  = 20 µm.