Table 1. Quantitative analysis of pollen germination rates and pollen tube lengths of the Lat52-GFP, Lat52-Ann5-GFP 1 and 3, and Ann5Pro-Ann5 1 homozygous lines.

	BFA (µM)	Lat52-GFP	Lat52-Ann5-GFP 1	Lat52-Ann5-GFP 2	Ann5Pro-Ann5 1
Pollen germination rate, %	0	89.7±1.5	89.4±2.3	92.6±1.1	91.8±1.6
	0.3	51.4±3.1	85.3±3.2**	68.7±3.2*	66.4±2.3*
	0.6	6.9±1.7	53.8±4.5**	23.3±3.3**	22.1±2.5**
Average pollen tube length, µm	0	361.7±10.4	353.3±16.8	352.8±9.7	349.0±10.1
	0.3	231.4±9.1	304.8±8.6**	271.5±9.3*	267.9±9.6*
	0.6	192.4±7.0	262.9±8.6**	235.2±7.5*	226.7±6.7*

For pollen germination rate, the pollen germinated for 3 h in the presence of 0.3 or 0.6 µM BFA. Eight hundred pollen grains and tubes were counted. For pollen tube length, the pollen germinated normally for 2 h and was then treated with 0.3 or 0.6 µM BFA for 2 h. A total of 500 pollen tubes were measured. Values represent the means ± _SD. *P<0.05 and **P<0.01 by Student's t test.