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. 2014 Jul 14;9(7):e102582. doi: 10.1371/journal.pone.0102582

Figure 1. Rb regulates oxidative stress in 3T3 cells.

Figure 1

(A) ROS levels were determined based on DCF or DHE fluorescence after cells were cultured for 24 h. (B and C) GSSG/GSH and NADP+/NADPH ratios were measured under the normal culture condition. (D) Cells expressing Grx1-roGFP2 were excited with 400 and 480 nm wavelengths, and the ratio of emission in the channel (535 nm) was calculated (right). Pseudocolor image was shown with the color scale (left). (E) ROS levels were determined based on DCF fluorescence after 3T3/Rb−/− cells with or without Rb re-expression were cultured for 24 h. (F) Ratios of fluorescence obtained at 400/480 nm excitation wavelengths were measured based on JC-1 fluorescence after 3T3/Rb−/− cells with or without Rb re-expression were cultured for 24 h. *P<0.05, **P<0.01.